#SampleID	BarcodeSequence	LinkerPrimerSequence	blank	center_name	center_project_name	control	experiment_design_description	extraction_robot	extractionkit_lot	instrument_model	library_construction_protocol	linker	mastermix_lot	orig_name	pcr_primers	platform	plating	primer_date	primer_plate	processing_robot	project_name	run_center	run_date	run_prefix	sample_plate	sequencing_meth	sputum	target_gene	target_subfragment	tm1000_8_tool	tm300_8_tool	tm50_8_tool	water_lot	well_description	well_id	wrongsample	anonymized_name	bubble_measure	bubbles	collection_timestamp	dna_extracted	elevation	env_biome	env_feature	env_material	env_package	geo_loc_name	healthy	host_subject_id	latitude	longitude	original_sample	ph	physical_specimen_location	physical_specimen_remaining	sample_number	sample_type	scientific_name	taxon_id	title	Description
10511.151.1.Spu	CTGAGGCCCTTC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	151.1.Spu	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate93	sequencing by synthesis	Yes	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate93_151.1.Spu_H7	H7	10511.151.1.Spu	5	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF151	32.7157	116.1611	10511.151.1.Spu	Not applicable	Dorrestein UCSDMI	TRUE	2	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF151_2
10511.151.1.Spu.pH5	CAGTCCCTGCAC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	151.1.Spu.pH5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate80	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate80_151.1.Spu.pH5_G6	G6	10511.151.1.Spu.pH5	6	per cent bubble in tube	0	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF151	32.7157	116.1611	10511.151.1.Spu	5	Dorrestein UCSDMI	TRUE	2	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF151_2
10511.151.1.Spu.pH5.5	CTCCACATTCCT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	151.1.Spu.pH5.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate67	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate67_151.1.Spu.pH5.5_F5	F5	10511.151.1.Spu.pH5.5	7	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF151	32.7157	116.1611	10511.151.1.Spu	5.5	Dorrestein UCSDMI	TRUE	2	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF151_2
10511.151.1.Spu.pH6	GTTGGTTGGCAT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	151.1.Spu.pH6	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate54	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate54_151.1.Spu.pH6_E4	E4	10511.151.1.Spu.pH6	8	per cent bubble in tube	0	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF151	32.7157	116.1611	10511.151.1.Spu	6	Dorrestein UCSDMI	TRUE	2	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF151_2
10511.151.1.Spu.pH6.5	TCCGCTGCTGAC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	151.1.Spu.pH6.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate41	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate41_151.1.Spu.pH6.5_D3	D3	10511.151.1.Spu.pH6.5	9	per cent bubble in tube	0	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF151	32.7157	116.1611	10511.151.1.Spu	6.5	Dorrestein UCSDMI	TRUE	2	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF151_2
10511.151.1.Spu.pH7	GTCGTTACCCGC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	151.1.Spu.pH7	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate27	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate27_151.1.Spu.pH7_C1	C1	10511.151.1.Spu.pH7	10	per cent bubble in tube	0	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF151	32.7157	116.1611	10511.151.1.Spu	7	Dorrestein UCSDMI	TRUE	2	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF151_2
10511.151.1.Spu.pH7.5	TCATCTTGATTG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	151.1.Spu.pH7.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_151.1.Spu.pH7.5_B12	B12	10511.151.1.Spu.pH7.5	11	per cent bubble in tube	0	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF151	32.7157	116.1611	10511.151.1.Spu	7.5	Dorrestein UCSDMI	TRUE	2	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF151_2
10511.151.1.Spu.pH8	AGGTGAGTTCTA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	151.1.Spu.pH8	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_151.1.Spu.pH8_A11	A11	10511.151.1.Spu.pH8	12	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF151	32.7157	116.1611	10511.151.1.Spu	8	Dorrestein UCSDMI	TRUE	2	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF151_2
10511.151.1.Spu.pH8.5	GGACCTGGATGG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	151.1.Spu.pH8.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_151.1.Spu.pH8.5_H9	H9	10511.151.1.Spu.pH8.5	13	per cent bubble in tube	0	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF151	32.7157	116.1611	10511.151.1.Spu	8.5	Dorrestein UCSDMI	TRUE	2	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF151_2
10511.151.Spu	CTGGTGCTGAAT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	151.Spu	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate46	sequencing by synthesis	Yes	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate46_151.Spu_D8	D8	10511.151.Spu	14	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF151	32.7157	116.1611	10511.151.Spu	Not applicable	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF151_1
10511.151.Spu.pH5	CATTCGTGGCGT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	151.Spu.pH5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate32	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate32_151.Spu.pH5_C6	C6	10511.151.Spu.pH5	15	per cent bubble in tube	0	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF151	32.7157	116.1611	10511.151.Spu	5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF151_1
10511.151.Spu.pH5.5	CTTCCAACTCAT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	151.Spu.pH5.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate19	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate19_151.Spu.pH5.5_B5	B5	10511.151.Spu.pH5.5	16	per cent bubble in tube	0	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF151	32.7157	116.1611	10511.151.Spu	5.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF151_1
10511.151.Spu.pH6	ATTTGGCTCTTA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	151.Spu.pH6	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate6	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate6_151.Spu.pH6_A4	A4	10511.151.Spu.pH6	17	per cent bubble in tube	0	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF151	32.7157	116.1611	10511.151.Spu	6	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF151_1
10511.151.Spu.pH6.5	ATAAACGGACAT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	151.Spu.pH6.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate87	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate87_151.Spu.pH6.5_H1	H1	10511.151.Spu.pH6.5	18	per cent bubble in tube	0	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF151	32.7157	116.1611	10511.151.Spu	6.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF151_1
10511.151.Spu.pH7	TCTTACCCATAA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	151.Spu.pH7	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_151.Spu.pH7_G12	G12	10511.151.Spu.pH7	19	per cent bubble in tube	0	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF151	32.7157	116.1611	10511.151.Spu	7	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF151_1
10511.151.Spu.pH7.5	CGATTCCTTAAT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	151.Spu.pH7.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_151.Spu.pH7.5_F11	F11	10511.151.Spu.pH7.5	20	per cent bubble in tube	0	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF151	32.7157	116.1611	10511.151.Spu	7.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF151_1
10511.151.Spu.pH8	ACCACCGTAACC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	151.Spu.pH8	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_151.Spu.pH8_E10	E10	10511.151.Spu.pH8	21	per cent bubble in tube	0	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF151	32.7157	116.1611	10511.151.Spu	8	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF151_1
10511.151.Spu.pH8.5	CGTCGCAGCCTT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	151.Spu.pH8.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_151.Spu.pH8.5_D9	D9	10511.151.Spu.pH8.5	22	per cent bubble in tube	0	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF151	32.7157	116.1611	10511.151.Spu	8.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF151_1
10511.160.2.Spu.pH5	GTTAAGCTGACC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	160.2.Spu.pH5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate8	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate8_160.2.Spu.pH5_A6	A6	10511.160.2.Spu.pH5	23	per cent bubble in tube	1.928852399	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF160	32.7157	116.1611	10511.160.Spu	5	Dorrestein UCSDMI	TRUE	2	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF160_2
10511.160.2.Spu.pH5.5	GGTGCAGACAGA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	160.2.Spu.pH5.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate90	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate90_160.2.Spu.pH5.5_H4	H4	10511.160.2.Spu.pH5.5	24	per cent bubble in tube	1.928852399	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF160	32.7157	116.1611	10511.160.Spu	5.5	Dorrestein UCSDMI	TRUE	2	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF160_2
10511.160.2.Spu.pH6.5	GACATCTGACAC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	160.2.Spu.pH6.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate63	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate63_160.2.Spu.pH6.5_F1	F1	10511.160.2.Spu.pH6.5	26	per cent bubble in tube	20.76889792	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF160	32.7157	116.1611	10511.160.Spu	6.5	Dorrestein UCSDMI	TRUE	2	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF160_2
10511.160.2.Spu.pH7	CGTTCGCTAGCC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	160.2.Spu.pH7	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_160.2.Spu.pH7_E12	E12	10511.160.2.Spu.pH7	27	per cent bubble in tube	34.64571271	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF160	32.7157	116.1611	10511.160.Spu	7	Dorrestein UCSDMI	TRUE	2	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF160_2
10511.160.2.Spu.pH7.5	CGCACGTACCTC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	160.2.Spu.pH7.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_160.2.Spu.pH7.5_D11	D11	10511.160.2.Spu.pH7.5	28	per cent bubble in tube	11.65787661	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF160	32.7157	116.1611	10511.160.Spu	7.5	Dorrestein UCSDMI	TRUE	2	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF160_2
10511.160.2.Spu.pH8	CGCTAGGATGTT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	160.2.Spu.pH8	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_160.2.Spu.pH8_C10	C10	10511.160.2.Spu.pH8	29	per cent bubble in tube	0	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF160	32.7157	116.1611	10511.160.Spu	8	Dorrestein UCSDMI	TRUE	2	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF160_2
10511.160.2.Spu.pH8.5	TCTAACGAGTGC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	160.2.Spu.pH8.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_160.2.Spu.pH8.5_B9	B9	10511.160.2.Spu.pH8.5	30	per cent bubble in tube	0	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF160	32.7157	116.1611	10511.160.Spu	8.5	Dorrestein UCSDMI	TRUE	2	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF160_2
10511.160.Spu	TATGGGTAGCTA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	160.Spu	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate94	sequencing by synthesis	Yes	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate94_160.Spu_H8	H8	10511.160.Spu	31	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF160	32.7157	116.1611	10511.160.Spu	Not applicable	Dorrestein UCSDMI	TRUE	2	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF160_2
10511.160.Spu.pH6	CACGAAAGCAGG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	160.Spu.pH6	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate77	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate77_160.Spu.pH6_G3	G3	10511.160.Spu.pH6	25	per cent bubble in tube	35.56487008	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF160	32.7157	116.1611	10511.160.Spu	6	Dorrestein UCSDMI	TRUE	2	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF160_2
10511.217	ATTTCCGCTAAT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	217	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	Yes	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_217_E11	E11	10511.217	1	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF217	32.7157	116.1611	10511.217	Not applicable	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF217
10511.217.PH.5.0	AACCACTAACCG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	217.PH_5.0	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_217.PH_5.0_A7	A7	10511.217.PH.5.0	32	per cent bubble in tube	8.796343652	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF217	32.7157	116.1611	10511.217	5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF217
10511.217.PH.5.5	CCGAGGTATAAT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	217.PH_5.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_217.PH_5.5_B7	B7	10511.217.PH.5.5	33	per cent bubble in tube	14.56472731	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF217	32.7157	116.1611	10511.217	5.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF217
10511.217.PH.6.0	TTAACAAGGCAA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	217.PH_6.0	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_217.PH_6.0_C7	C7	10511.217.PH.6.0	34	per cent bubble in tube	18.60924003	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF217	32.7157	116.1611	10511.217	6	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF217
10511.217.PH.6.5	AAGTCACACACA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	217.PH_6.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_217.PH_6.5_D7	D7	10511.217.PH.6.5	35	per cent bubble in tube	22.08555801	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF217	32.7157	116.1611	10511.217	6.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF217
10511.217.PH.7.0	TGGCAGCGAGCC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	217.PH_7.0	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_217.PH_7.0_E7	E7	10511.217.PH.7.0	36	per cent bubble in tube	0	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF217	32.7157	116.1611	10511.217	7	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF217
10511.217.PH.7.5	CCGGCTTATGTG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	217.PH_7.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_217.PH_7.5_F7	F7	10511.217.PH.7.5	37	per cent bubble in tube	5.09222186	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF217	32.7157	116.1611	10511.217	7.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF217
10511.217.PH.8.0	CGAATACTGACA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	217.PH_8.0	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_217.PH_8.0_G7	G7	10511.217.PH.8.0	38	per cent bubble in tube	0	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF217	32.7157	116.1611	10511.217	8	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF217
10511.217.PH.8.5	ACACACCCTGAC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	217.PH_8.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_217.PH_8.5_H7	H7	10511.217.PH.8.5	39	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF217	32.7157	116.1611	10511.217	8.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF217
10511.234	GTACTCGAACCA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	234	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	Yes	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_234_F11	F11	10511.234	2	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF234	32.7157	116.1611	10511.234	Not applicable	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF234
10511.234.PH.5.0	AACTTTCAGGAG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	234.PH_5.0	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_234.PH_5.0_A8	A8	10511.234.PH.5.0	40	per cent bubble in tube	5.253339	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF234	32.7157	116.1611	10511.234	5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF234
10511.234.PH.5.5	AGATTCGCTCGA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	234.PH_5.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_234.PH_5.5_B8	B8	10511.234.PH.5.5	41	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF234	32.7157	116.1611	10511.234	5.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF234
10511.234.PH.6.0	AACCGCATAAGT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	234.PH_6.0	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_234.PH_6.0_C8	C8	10511.234.PH.6.0	42	per cent bubble in tube	19.61361689	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF234	32.7157	116.1611	10511.234	6	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF234
10511.234.PH.6.5	GAAGTAGCGAGC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	234.PH_6.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_234.PH_6.5_D8	D8	10511.234.PH.6.5	43	per cent bubble in tube	17.11634187	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF234	32.7157	116.1611	10511.234	6.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF234
10511.234.PH.7.0	GTGAATGTTCGA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	234.PH_7.0	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_234.PH_7.0_E8	E8	10511.234.PH.7.0	44	per cent bubble in tube	21.85786922	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF234	32.7157	116.1611	10511.234	7	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF234
10511.234.PH.7.5	ATAGTCCTTTAA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	234.PH_7.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_234.PH_7.5_F8	F8	10511.234.PH.7.5	45	per cent bubble in tube	10.65723031	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF234	32.7157	116.1611	10511.234	7.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF234
10511.234.PH.8.0	TCTTATAACGCT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	234.PH_8.0	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_234.PH_8.0_G8	G8	10511.234.PH.8.0	46	per cent bubble in tube	0	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF234	32.7157	116.1611	10511.234	8	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF234
10511.234.PH.8.5	TATAGGCTCCGC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	234.PH_8.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_234.PH_8.5_H8	H8	10511.234.PH.8.5	47	per cent bubble in tube	0	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF234	32.7157	116.1611	10511.234	8.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF234
10511.301.Spu	CAGTGCACGTCT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	39.Spu	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate34	sequencing by synthesis	Yes	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate34_39.Spu_C8	C8	10511.39.Spu	90	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF301	32.7157	116.1611	10511.301.Spu	Not applicable	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF301
10511.301.Spu.pH5	GCCTAGCCCAAT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	301.Spu.pH5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate20	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate20_301.Spu.pH5_B6	B6	10511.301.Spu.pH5	48	per cent bubble in tube	6.852476894	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF301	32.7157	116.1611	10511.301.Spu	5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF301
10511.301.Spu.pH5.5	GATCCGGCAGGA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	301.Spu.pH5.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate7	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate7_301.Spu.pH5.5_A5	A5	10511.301.Spu.pH5.5	49	per cent bubble in tube	7.531967914	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF301	32.7157	116.1611	10511.301.Spu	5.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF301
10511.301.Spu.pH6	CGTGGCTTTCCG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	301.Spu.pH6	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate89	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate89_301.Spu.pH6_H3	H3	10511.301.Spu.pH6	50	per cent bubble in tube	9.500024954	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF301	32.7157	116.1611	10511.301.Spu	6	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF301
10511.301.Spu.pH6.5	AATTTCCTAACA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	301.Spu.pH6.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate75	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate75_301.Spu.pH6.5_G1	G1	10511.301.Spu.pH6.5	51	per cent bubble in tube	19.55280166	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF301	32.7157	116.1611	10511.301.Spu	6.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF301
10511.301.Spu.pH7	TGCCTGCTCGAC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	301.Spu.pH7	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_301.Spu.pH7_F12	F12	10511.301.Spu.pH7	52	per cent bubble in tube	6.098681083	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF301	32.7157	116.1611	10511.301.Spu	7	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF301
10511.301.Spu.pH7.5	AGGAAGTAACTT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	301.Spu.pH7.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_301.Spu.pH7.5_E11	E11	10511.301.Spu.pH7.5	53	per cent bubble in tube	2.748353851	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF301	32.7157	116.1611	10511.301.Spu	7.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF301
10511.301.Spu.pH8	CTACTTACATCC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	301.Spu.pH8	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_301.Spu.pH8_D10	D10	10511.301.Spu.pH8	54	per cent bubble in tube	0.510855683	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF301	32.7157	116.1611	10511.301.Spu	8	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF301
10511.301.Spu.pH8.5	CGTTCCTTGTTA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	301.Spu.pH8.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_301.Spu.pH8.5_C9	C9	10511.301.Spu.pH8.5	55	per cent bubble in tube	0	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF301	32.7157	116.1611	10511.301.Spu	8.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF301
10511.305	ACACCGCACAAT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	305	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	Yes	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_305_G11	G11	10511.305	3	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF305	32.7157	116.1611	10511.305	Not applicable	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF305
10511.305.PH.5.0	CCAGGACAGGAA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	305.PH_5.0	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_305.PH_5.0_A9	A9	10511.305.PH.5.0	56	per cent bubble in tube	13.95189289	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF305	32.7157	116.1611	10511.305	5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF305
10511.305.PH.5.5	TTGCCGCTCTGG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	305.PH_5.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_305.PH_5.5_B9	B9	10511.305.PH.5.5	57	per cent bubble in tube	14.48576788	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF305	32.7157	116.1611	10511.305	5.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF305
10511.305.PH.6.0	CCACAACGATCA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	305.PH_6.0	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_305.PH_6.0_C9	C9	10511.305.PH.6.0	58	per cent bubble in tube	29.22665995	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF305	32.7157	116.1611	10511.305	6	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF305
10511.305.PH.6.5	CACCATCTCCGG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	305.PH_6.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_305.PH_6.5_D9	D9	10511.305.PH.6.5	59	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF305	32.7157	116.1611	10511.305	6.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF305
10511.305.PH.7.0	TATGTTGACGGC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	305.PH_7.0	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_305.PH_7.0_E9	E9	10511.305.PH.7.0	60	per cent bubble in tube	15.20895392	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF305	32.7157	116.1611	10511.305	7	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF305
10511.305.PH.7.5	TCGAGCCGATCT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	305.PH_7.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_305.PH_7.5_F9	F9	10511.305.PH.7.5	61	per cent bubble in tube	13.86713066	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF305	32.7157	116.1611	10511.305	7.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF305
10511.305.PH.8.0	TAAGGTCGATAA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	305.PH_8.0	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_305.PH_8.0_G9	G9	10511.305.PH.8.0	62	per cent bubble in tube	3.323834461	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF305	32.7157	116.1611	10511.305	8	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF305
10511.305.PH.8.5	ATAATTGCCGAG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	305.PH_8.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_305.PH_8.5_H9	H9	10511.305.PH.8.5	63	per cent bubble in tube	0	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF305	32.7157	116.1611	10511.305	8.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF305
10511.34.Spu	TATACCGCTGCG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	34.Spu	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate22	sequencing by synthesis	Yes	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate22_34.Spu_B8	B8	10511.34.Spu	64	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF34	32.7157	116.1611	10511.34.Spu	Not applicable	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF34
10511.34.Spu.pH5	GCGGTCGGTCAG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	34.Spu.pH5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate44	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate44_34.Spu.pH5_D6	D6	10511.34.Spu.pH5	65	per cent bubble in tube	0	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF34	32.7157	116.1611	10511.34.Spu	5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF34
10511.34.Spu.pH5.5	CGACCTCGCATA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	34.Spu.pH5.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate31	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate31_34.Spu.pH5.5_C5	C5	10511.34.Spu.pH5.5	66	per cent bubble in tube	1.496730348	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF34	32.7157	116.1611	10511.34.Spu	5.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF34
10511.34.Spu.pH6	CGCGTCAAACTA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	34.Spu.pH6	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate18	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate18_34.Spu.pH6_B4	B4	10511.34.Spu.pH6	67	per cent bubble in tube	11.57531446	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF34	32.7157	116.1611	10511.34.Spu	6	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF34
10511.34.Spu.pH6.5	TCATCCGTCGGC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	34.Spu.pH6.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate5	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate5_34.Spu.pH6.5_A3	A3	10511.34.Spu.pH6.5	68	per cent bubble in tube	9.306700853	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF34	32.7157	116.1611	10511.34.Spu	6.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF34
10511.34.Spu.pH7	TGTGCTTGTAGG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	34.Spu.pH7	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_34.Spu.pH7_H12	H12	10511.34.Spu.pH7	69	per cent bubble in tube	27.59978243	11/30/2015	TRUE	101	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF34	32.7157	116.1611	10511.34.Spu	7	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF34
10511.34.Spu.pH7.5	AATCCGGTCACC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	34.Spu.pH7.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_34.Spu.pH7.5_G11	G11	10511.34.Spu.pH7.5	70	per cent bubble in tube	17.90344241	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF34	32.7157	116.1611	10511.34.Spu	7.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF34
10511.34.Spu.pH8	CGGTTGGCGGGT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	34.Spu.pH8	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_34.Spu.pH8_F10	F10	10511.34.Spu.pH8	71	per cent bubble in tube	5.805781618	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF34	32.7157	116.1611	10511.34.Spu	8	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF34
10511.34.Spu.pH8.5	AGCGATTCCTCG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	34.Spu.pH8.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_34.Spu.pH8.5_E9	E9	10511.34.Spu.pH8.5	72	per cent bubble in tube	0.496585971	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF34	32.7157	116.1611	10511.34.Spu	8.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF34
10511.353	CAGCCCTACCCA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	353	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	Yes	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_353_H11	H11	10511.353	4	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF353	32.7157	116.1611	10511.353	Not applicable	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF353
10511.353.PH.5.0	GCGCGGCGTTGC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	353.PH_5.0	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_353.PH_5.0_A10	A10	10511.353.PH.5.0	73	per cent bubble in tube	8.526021888	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF353	32.7157	116.1611	10511.353	5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF353
10511.353.PH.5.5	AGACTTCTCAGG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	353.PH_5.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_353.PH_5.5_B10	B10	10511.353.PH.5.5	74	per cent bubble in tube	8.607786951	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF353	32.7157	116.1611	10511.353	5.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF353
10511.353.PH.6.0	AGTTCTCATTAA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	353.PH_6.0	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_353.PH_6.0_C10	C10	10511.353.PH.6.0	75	per cent bubble in tube	7.527276802	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF353	32.7157	116.1611	10511.353	6	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF353
10511.353.PH.6.5	GTGTCGAGGGCA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	353.PH_6.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_353.PH_6.5_D10	D10	10511.353.PH.6.5	76	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF353	32.7157	116.1611	10511.353	6.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF353
10511.353.PH.7.0	AGTGTTTCGGAC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	353.PH_7.0	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_353.PH_7.0_E10	E10	10511.353.PH.7.0	77	per cent bubble in tube	19.50462255	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF353	32.7157	116.1611	10511.353	7	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF353
10511.353.PH.7.5	AGTGCAGGAGCC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	353.PH_7.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_353.PH_7.5_F10	F10	10511.353.PH.7.5	78	per cent bubble in tube	1.53168396	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF353	32.7157	116.1611	10511.353	7.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF353
10511.353.PH.8.0	GTTGCTGAGTCC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	353.PH_8.0	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_353.PH_8.0_G10	G10	10511.353.PH.8.0	79	per cent bubble in tube	0	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF353	32.7157	116.1611	10511.353	8	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF353
10511.353.PH.8.5	CGGAGAGACATG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	353.PH_8.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_353.PH_8.5_H10	H10	10511.353.PH.8.5	80	per cent bubble in tube	0	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF353	32.7157	116.1611	10511.353	8.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF353
10511.356.Spu	ACAAAGGTATCA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	356.Spu	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate58	sequencing by synthesis	Yes	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate58_356.Spu_E8	E8	10511.356.Spu	81	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF356	32.7157	116.1611	10511.356.Spu	Not applicable	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF356
10511.356.Spu.pH5	CGAACGTCTATG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	356.Spu.pH5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate68	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate68_356.Spu.pH5_F6	F6	10511.356.Spu.pH5	82	per cent bubble in tube	4.264890259	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF356	32.7157	116.1611	10511.356.Spu	5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF356
10511.356.Spu.pH5.5	GAACGATCATGT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	356.Spu.pH5.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate55	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate55_356.Spu.pH5.5_E5	E5	10511.356.Spu.pH5.5	83	per cent bubble in tube	19.61919269	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF356	32.7157	116.1611	10511.356.Spu	5.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF356
10511.356.Spu.pH6	TCCGTGGTATAG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	356.Spu.pH6	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate42	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate42_356.Spu.pH6_D4	D4	10511.356.Spu.pH6	84	per cent bubble in tube	45.74674318	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF356	32.7157	116.1611	10511.356.Spu	6	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF356
10511.356.Spu.pH6.5	CGGTCAATTGAC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	356.Spu.pH6.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate29	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate29_356.Spu.pH6.5_C3	C3	10511.356.Spu.pH6.5	85	per cent bubble in tube	42.59072581	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF356	32.7157	116.1611	10511.356.Spu	6.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF356
10511.356.Spu.pH7	AAGTGGCTATCC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	356.Spu.pH7	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate15	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate15_356.Spu.pH7_B1	B1	10511.356.Spu.pH7	86	per cent bubble in tube	4.821672839	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF356	32.7157	116.1611	10511.356.Spu	7	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF356
10511.356.Spu.pH7.5	AACGATCATAGA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	356.Spu.pH7.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_356.Spu.pH7.5_A12	A12	10511.356.Spu.pH7.5	87	per cent bubble in tube	3.618649965	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF356	32.7157	116.1611	10511.356.Spu	7.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF356
10511.356.Spu.pH8	TATGTGCCGGCT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	356.Spu.pH8	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_356.Spu.pH8_H10	H10	10511.356.Spu.pH8	88	per cent bubble in tube	3.434817162	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF356	32.7157	116.1611	10511.356.Spu	8	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF356
10511.356.Spu.pH8.5	AGAGACGCGTAG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	356.Spu.pH8.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_356.Spu.pH8.5_G9	G9	10511.356.Spu.pH8.5	89	per cent bubble in tube	0	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF356	32.7157	116.1611	10511.356.Spu	8.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF356
10511.46.Spu	ATCAAGATACGC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	46.Spu	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate81	sequencing by synthesis	Yes	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate81_46.Spu_G7	G7	10511.46.Spu	91	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF46	32.7157	116.1611	10511.46.Spu	Not applicable	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF46
10511.46.Spu.pH5	TGATTTAATTGC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	46.Spu.pH5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate92	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate92_46.Spu.pH5_H6	H6	10511.46.Spu.pH5	92	per cent bubble in tube	0.759493671	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF46	32.7157	116.1611	10511.46.Spu	5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF46
10511.46.Spu.pH5.5	TCTTCGCAGCAG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	46.Spu.pH5.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate79	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate79_46.Spu.pH5.5_G5	G5	10511.46.Spu.pH5.5	93	per cent bubble in tube	2.386666237	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF46	32.7157	116.1611	10511.46.Spu	5.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF46
10511.46.Spu.pH6	AACGGGCGACGT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	46.Spu.pH6	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate66	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate66_46.Spu.pH6_F4	F4	10511.46.Spu.pH6	94	per cent bubble in tube	2.64039729	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF46	32.7157	116.1611	10511.46.Spu	6	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF46
10511.46.Spu.pH6.5	TGCGCGCCTTCC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	46.Spu.pH6.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate53	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate53_46.Spu.pH6.5_E3	E3	10511.46.Spu.pH6.5	95	per cent bubble in tube	6.02739726	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF46	32.7157	116.1611	10511.46.Spu	6.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF46
10511.46.Spu.pH7	AGTATATGTTTC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	46.Spu.pH7	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate39	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate39_46.Spu.pH7_D1	D1	10511.46.Spu.pH7	96	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF46	32.7157	116.1611	10511.46.Spu	7	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF46
10511.46.Spu.pH7.5	GTCAGTATGGCT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	46.Spu.pH7.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_46.Spu.pH7.5_C12	C12	10511.46.Spu.pH7.5	97	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF46	32.7157	116.1611	10511.46.Spu	7.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF46
10511.46.Spu.pH8	CCGCCAGCTTTG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	46.Spu.pH8	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_46.Spu.pH8_B11	B11	10511.46.Spu.pH8	98	per cent bubble in tube	0	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF46	32.7157	116.1611	10511.46.Spu	8	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF46
10511.46.Spu.pH8.5	TGCTACAGACGT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	46.Spu.pH8.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_46.Spu.pH8.5_A10	A10	10511.46.Spu.pH8.5	99	per cent bubble in tube	0	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF46	32.7157	116.1611	10511.46.Spu	8.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF46
10511.53.Spu	CAATAGAATAAG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	53.Spu	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate10	sequencing by synthesis	Yes	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate10_53.Spu_A8	A8	10511.53.Spu	100	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF53	32.7157	116.1611	10511.53.Spu	Not applicable	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF53
10511.53.Spu.pH5	TCAGTCAGATGA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	53.Spu.pH5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate56	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate56_53.Spu.pH5_E6	E6	10511.53.Spu.pH5	101	per cent bubble in tube	1.586140184	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF53	32.7157	116.1611	10511.53.Spu	5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF53
10511.53.Spu.pH5.5	GTCAGAGTATTG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	53.Spu.pH5.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate43	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate43_53.Spu.pH5.5_D5	D5	10511.53.Spu.pH5.5	102	per cent bubble in tube	13.65892705	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF53	32.7157	116.1611	10511.53.Spu	5.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF53
10511.53.Spu.pH6	ATGTTCCTCATC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	53.Spu.pH6	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate30	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate30_53.Spu.pH6_C4	C4	10511.53.Spu.pH6	103	per cent bubble in tube	13.16926019	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF53	32.7157	116.1611	10511.53.Spu	6	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF53
10511.53.Spu.pH6.5	GGCTTCGGAGCG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	53.Spu.pH6.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate17	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate17_53.Spu.pH6.5_B3	B3	10511.53.Spu.pH6.5	104	per cent bubble in tube	12.66164911	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF53	32.7157	116.1611	10511.53.Spu	6.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF53
10511.53.Spu.pH7	ATGTTAGGGAAT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	53.Spu.pH7	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate3	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate3_53.Spu.pH7_A1	A1	10511.53.Spu.pH7	105	per cent bubble in tube	2.272727273	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF53	32.7157	116.1611	10511.53.Spu	7	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF53
10511.53.Spu.pH7.5	ATACCGTCTTTC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	53.Spu.pH7.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_53.Spu.pH7.5_H11	H11	10511.53.Spu.pH7.5	106	per cent bubble in tube	0	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF53	32.7157	116.1611	10511.53.Spu	7.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF53
10511.53.Spu.pH8	CCACCGGGCCGA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	53.Spu.pH8	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_53.Spu.pH8_G10	G10	10511.53.Spu.pH8	107	per cent bubble in tube	0	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF53	32.7157	116.1611	10511.53.Spu	8	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF53
10511.53.Spu.pH8.5	CGTGAGGACCAG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	53.Spu.pH8.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_53.Spu.pH8.5_F9	F9	10511.53.Spu.pH8.5	108	per cent bubble in tube	0	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF53	32.7157	116.1611	10511.53.Spu	8.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF53
10511.82.Spu	AATAAGCAATAG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	82.Spu	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate82	sequencing by synthesis	Yes	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate82_82.Spu_G8	G8	10511.82.Spu	109	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF82	32.7157	116.1611	10511.82.Spu	Not applicable	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF82
10511.82.Spu.pH5	GTAATTGTAATT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	82.Spu.pH5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_82.Spu.pH5_A9	A9	10511.82.Spu.pH5	110	per cent bubble in tube	1.928852399	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF82	32.7157	116.1611	10511.82.Spu	5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF82
10511.82.Spu.pH5.5	GATCCCACGTAC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	82.Spu.pH5.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_82.Spu.pH5.5_B10	B10	10511.82.Spu.pH5.5	111	per cent bubble in tube	10.75438688	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF82	32.7157	116.1611	10511.82.Spu	5.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF82
10511.82.Spu.pH6	AGCGGTAGCGGT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	82.Spu.pH6	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_82.Spu.pH6_C11	C11	10511.82.Spu.pH6	112	per cent bubble in tube	13.91082595	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF82	32.7157	116.1611	10511.82.Spu	6	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF82
10511.82.Spu.pH6.5	GTCCTCGCGACT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	82.Spu.pH6.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_82.Spu.pH6.5_D12	D12	10511.82.Spu.pH6.5	113	per cent bubble in tube	13.21147411	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF82	32.7157	116.1611	10511.82.Spu	6.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF82
10511.82.Spu.pH7	GGCTCGTCGGAG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	82.Spu.pH7	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate51	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate51_82.Spu.pH7_E1	E1	10511.82.Spu.pH7	114	per cent bubble in tube	10.02763285	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF82	32.7157	116.1611	10511.82.Spu	7	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF82
10511.82.Spu.pH7.5	GCGCACACCTTC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	82.Spu.pH7.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate65	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate65_82.Spu.pH7.5_F3	F3	10511.82.Spu.pH7.5	115	per cent bubble in tube	7.422918077	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF82	32.7157	116.1611	10511.82.Spu	7.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF82
10511.82.Spu.pH8	TACTGAGCCTCG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	82.Spu.pH8	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate78	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate78_82.Spu.pH8_G4	G4	10511.82.Spu.pH8	116	per cent bubble in tube	0	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF82	32.7157	116.1611	10511.82.Spu	8	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF82
10511.82.Spu.pH8.5	CACGCTATTGGA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	82.Spu.pH8.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate91	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate91_82.Spu.pH8.5_H5	H5	10511.82.Spu.pH8.5	117	per cent bubble in tube	0	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF82	32.7157	116.1611	10511.82.Spu	8.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF82
10511.95.Spu	TGAACCCTATGG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	95.Spu	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate70	sequencing by synthesis	Yes	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate70_95.Spu_F8	F8	10511.95.Spu	118	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF95	32.7157	116.1611	10511.95.Spu	Not applicable	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF95
10511.95.Spu.pH5	ACTTAGACTCTT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	95.Spu.pH5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_95.Spu.pH5_H8	H8	10511.95.Spu.pH5	119	per cent bubble in tube	34.335	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF95	32.7157	116.1611	10511.95.Spu	5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF95
10511.95.Spu.pH5.5	GCTGAGCCTTTG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	95.Spu.pH5.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_95.Spu.pH5.5_G8	G8	10511.95.Spu.pH5.5	120	per cent bubble in tube	41.91440537	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF95	32.7157	116.1611	10511.95.Spu	5.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF95
10511.95.Spu.pH6	AGTCCGAGTTGT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	95.Spu.pH6	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_95.Spu.pH6_F8	F8	10511.95.Spu.pH6	121	per cent bubble in tube	43.02821971	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF95	32.7157	116.1611	10511.95.Spu	6	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF95
10511.95.Spu.pH6.5	AGACTATTTCAT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	95.Spu.pH6.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_95.Spu.pH6.5_E8	E8	10511.95.Spu.pH6.5	122	per cent bubble in tube	45.57499115	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF95	32.7157	116.1611	10511.95.Spu	6.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF95
10511.95.Spu.pH7	ACACGTTTGGGT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	95.Spu.pH7	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_95.Spu.pH7_D8	D8	10511.95.Spu.pH7	123	per cent bubble in tube	45.94577459	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF95	32.7157	116.1611	10511.95.Spu	7	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF95
10511.95.Spu.pH7.5	CCATGTGGCTCC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	95.Spu.pH7.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_95.Spu.pH7.5_C8	C8	10511.95.Spu.pH7.5	124	per cent bubble in tube	37.67172813	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF95	32.7157	116.1611	10511.95.Spu	7.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF95
10511.95.Spu.pH8	ACGTTAATATTC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	95.Spu.pH8	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_95.Spu.pH8_B8	B8	10511.95.Spu.pH8	125	per cent bubble in tube	0.422427035	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF95	32.7157	116.1611	10511.95.Spu	8	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF95
10511.95.Spu.pH8.5	GATAGCACTCGT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	95.Spu.pH8.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_95.Spu.pH8.5_A8	A8	10511.95.Spu.pH8.5	126	per cent bubble in tube	0.712845622	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF95	32.7157	116.1611	10511.95.Spu	8.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ1.12A	TCCGCCTAGTCG	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ1.12A	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_BLANK.RQ1.12A_A12	A12	10511.BLANK.RQ1.12A	127	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ1.12A	32.7157	116.1611	BLANK.RQ1.12A	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ1.12B	CTATCTCCTGTC	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ1.12B	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_BLANK.RQ1.12B_B12	B12	10511.BLANK.RQ1.12B	128	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ1.12B	32.7157	116.1611	BLANK.RQ1.12B	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ1.12C	GATATACCAGTG	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ1.12C	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_BLANK.RQ1.12C_C12	C12	10511.BLANK.RQ1.12C	129	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ1.12C	32.7157	116.1611	BLANK.RQ1.12C	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ1.12D	AGAATCCACCAC	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ1.12D	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_BLANK.RQ1.12D_D12	D12	10511.BLANK.RQ1.12D	130	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ1.12D	32.7157	116.1611	BLANK.RQ1.12D	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ1.12E	CAAACCTATGGC	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ1.12E	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_BLANK.RQ1.12E_E12	E12	10511.BLANK.RQ1.12E	131	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ1.12E	32.7157	116.1611	BLANK.RQ1.12E	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ1.12F	ATAGGAATAACC	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ1.12F	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_BLANK.RQ1.12F_F12	F12	10511.BLANK.RQ1.12F	132	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ1.12F	32.7157	116.1611	BLANK.RQ1.12F	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ1.12G	CACAACCACAAC	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ1.12G	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_BLANK.RQ1.12G_G12	G12	10511.BLANK.RQ1.12G	133	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ1.12G	32.7157	116.1611	BLANK.RQ1.12G	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ1.12H	TCGTTGGGACTA	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ1.12H	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_BLANK.RQ1.12H_H12	H12	10511.BLANK.RQ1.12H	134	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ1.12H	32.7157	116.1611	BLANK.RQ1.12H	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ1.1A	CCTCGCATGACC	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ1.1A	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_BLANK.RQ1.1A_A1	A1	10511.BLANK.RQ1.1A	135	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ1.1A	32.7157	116.1611	BLANK.RQ1.1A	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ1.1B	CGCGCAAGTATT	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ1.1B	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_BLANK.RQ1.1B_B1	B1	10511.BLANK.RQ1.1B	136	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ1.1B	32.7157	116.1611	BLANK.RQ1.1B	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ1.1C	AAGGCGCTCCTT	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ1.1C	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_BLANK.RQ1.1C_C1	C1	10511.BLANK.RQ1.1C	137	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ1.1C	32.7157	116.1611	BLANK.RQ1.1C	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ1.1D	CGCAATGAGGGA	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ1.1D	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_BLANK.RQ1.1D_D1	D1	10511.BLANK.RQ1.1D	138	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ1.1D	32.7157	116.1611	BLANK.RQ1.1D	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ1.1E	ACGGCGTTATGT	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ1.1E	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_BLANK.RQ1.1E_E1	E1	10511.BLANK.RQ1.1E	139	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ1.1E	32.7157	116.1611	BLANK.RQ1.1E	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ1.1F	CATTTGACGACG	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ1.1F	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_BLANK.RQ1.1F_F1	F1	10511.BLANK.RQ1.1F	140	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ1.1F	32.7157	116.1611	BLANK.RQ1.1F	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ1.1G	GCTGCGTATACC	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ1.1G	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_BLANK.RQ1.1G_G1	G1	10511.BLANK.RQ1.1G	141	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ1.1G	32.7157	116.1611	BLANK.RQ1.1G	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ1.1H	GAGAAGCTTATA	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ1.1H	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_BLANK.RQ1.1H_H1	H1	10511.BLANK.RQ1.1H	142	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ1.1H	32.7157	116.1611	BLANK.RQ1.1H	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ2.1A	TAGGACGGGAGT	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ2.1A	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_BLANK.RQ2.1A_A1	A1	10511.BLANK.RQ2.1A	143	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ2.1A	32.7157	116.1611	BLANK.RQ2.1A	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ2.1B	GTTTGGCCACAC	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ2.1B	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_BLANK.RQ2.1B_B1	B1	10511.BLANK.RQ2.1B	144	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ2.1B	32.7157	116.1611	BLANK.RQ2.1B	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ2.1C	TATATAGTATCC	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ2.1C	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_BLANK.RQ2.1C_C1	C1	10511.BLANK.RQ2.1C	145	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ2.1C	32.7157	116.1611	BLANK.RQ2.1C	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ2.1D	CATAAGGGAGGC	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ2.1D	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_BLANK.RQ2.1D_D1	D1	10511.BLANK.RQ2.1D	146	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ2.1D	32.7157	116.1611	BLANK.RQ2.1D	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ2.1E	GTGCAACCAATC	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ2.1E	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_BLANK.RQ2.1E_E1	E1	10511.BLANK.RQ2.1E	147	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ2.1E	32.7157	116.1611	BLANK.RQ2.1E	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ2.1F	CTCACCTAGGAA	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ2.1F	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_BLANK.RQ2.1F_F1	F1	10511.BLANK.RQ2.1F	148	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ2.1F	32.7157	116.1611	BLANK.RQ2.1F	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ2.1G	TACTGTACTGTT	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ2.1G	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_BLANK.RQ2.1G_G1	G1	10511.BLANK.RQ2.1G	149	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ2.1G	32.7157	116.1611	BLANK.RQ2.1G	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ2.1H	CTAGAGCTCCCA	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ2.1H	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_BLANK.RQ2.1H_H1	H1	10511.BLANK.RQ2.1H	150	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ2.1H	32.7157	116.1611	BLANK.RQ2.1H	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ3.2A	GCTAGTTATGGA	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ3.2A	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate4	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate4_BLANK.RQ3.2A_A2	A2	10511.BLANK.RQ3.2A	151	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ3.2A	32.7157	116.1611	BLANK.RQ3.2A	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ3.2B	ACTAGTTGGACC	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ3.2B	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate16	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate16_BLANK.RQ3.2B_B2	B2	10511.BLANK.RQ3.2B	152	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ3.2B	32.7157	116.1611	BLANK.RQ3.2B	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ3.2C	CGCAAGCCCGCG	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ3.2C	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate28	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate28_BLANK.RQ3.2C_C2	C2	10511.BLANK.RQ3.2C	153	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ3.2C	32.7157	116.1611	BLANK.RQ3.2C	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ3.2D	AGAGCGGAACAA	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ3.2D	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate40	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate40_BLANK.RQ3.2D_D2	D2	10511.BLANK.RQ3.2D	154	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ3.2D	32.7157	116.1611	BLANK.RQ3.2D	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ3.2E	ACATGGGCGGAA	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ3.2E	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate52	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate52_BLANK.RQ3.2E_E2	E2	10511.BLANK.RQ3.2E	155	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ3.2E	32.7157	116.1611	BLANK.RQ3.2E	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ3.2F	CTGCGGATATAC	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ3.2F	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate64	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate64_BLANK.RQ3.2F_F2	F2	10511.BLANK.RQ3.2F	156	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ3.2F	32.7157	116.1611	BLANK.RQ3.2F	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ3.2G	GTGAGGGCAAGT	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ3.2G	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate76	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate76_BLANK.RQ3.2G_G2	G2	10511.BLANK.RQ3.2G	157	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ3.2G	32.7157	116.1611	BLANK.RQ3.2G	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ3.2H	ATATTGGCAGCC	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ3.2H	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate88	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate88_BLANK.RQ3.2H_H2	H2	10511.BLANK.RQ3.2H	158	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ3.2H	32.7157	116.1611	BLANK.RQ3.2H	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ4.3B	ACTCGCTCGCTG	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B24	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ4.3B	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	1	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate4	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate4_BLANK.RQ4.3B_B3	B3	10511.BLANK.RQ4.3B	159	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ4.3B	32.7157	116.1611	BLANK.RQ4.3B	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ4.3C	CTGTCTATACTA	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B24	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ4.3C	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	1	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate4	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate4_BLANK.RQ4.3C_C3	C3	10511.BLANK.RQ4.3C	160	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ4.3C	32.7157	116.1611	BLANK.RQ4.3C	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ4.3D	TAATCTCGCCGG	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B24	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ4.3D	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	1	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate4	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate4_BLANK.RQ4.3D_D3	D3	10511.BLANK.RQ4.3D	161	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ4.3D	32.7157	116.1611	BLANK.RQ4.3D	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ4.3E	AGTAAAGATCGT	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B24	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ4.3E	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	1	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate4	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate4_BLANK.RQ4.3E_E3	E3	10511.BLANK.RQ4.3E	162	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ4.3E	32.7157	116.1611	BLANK.RQ4.3E	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ4.3F	TTGCTGGACGCT	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B24	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ4.3F	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	1	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate4	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate4_BLANK.RQ4.3F_F3	F3	10511.BLANK.RQ4.3F	163	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ4.3F	32.7157	116.1611	BLANK.RQ4.3F	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ4.3G	TTGCGGACCCTA	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B24	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ4.3G	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	1	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate4	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate4_BLANK.RQ4.3G_G3	G3	10511.BLANK.RQ4.3G	164	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ4.3G	32.7157	116.1611	BLANK.RQ4.3G	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.BLANK.RQ4.3H	CGGTATAGCAAT	GTGTGCCAGCMGCCGCGGTAA	Yes	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B24	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	BLANK.RQ4.3H	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	1	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate4	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate4_BLANK.RQ4.3H_H3	H3	10511.BLANK.RQ4.3H	165	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	sterile water	misc environment	USA:CA:San Diego	Not applicable	BLANK.RQ4.3H	32.7157	116.1611	BLANK.RQ4.3H	Not applicable	Dorrestein UCSDMI	TRUE	1	control blank	metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	Blank
10511.C.PH.5.0	GGCGTAACGGCA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	Yes	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	C.PH_5.0	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_C.PH_5.0_A2	A2	10511.C.PH.5.0	166	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Not applicable	C	32.7157	116.1611	10511.C.PH.5.0	5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CON
10511.C.PH.5.5	AATACAGACCTG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	Yes	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	C.PH_5.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_C.PH_5.5_B2	B2	10511.C.PH.5.5	167	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Not applicable	C	32.7157	116.1611	10511.C.PH.5.5	5.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CON
10511.C.PH.6.0	GATCTAATCGAG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	Yes	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	C.PH_6.0	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_C.PH_6.0_C2	C2	10511.C.PH.6.0	168	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Not applicable	C	32.7157	116.1611	10511.C.PH.6.0	6	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CON
10511.C.PH.6.5	CCGCAGCCGCAG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	Yes	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	C.PH_6.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_C.PH_6.5_D2	D2	10511.C.PH.6.5	169	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Not applicable	C	32.7157	116.1611	10511.C.PH.6.5	6.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CON
10511.C.PH.7.0	GAACCGTGCAGG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	Yes	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	C.PH_7.0	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_C.PH_7.0_E2	E2	10511.C.PH.7.0	170	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Not applicable	C	32.7157	116.1611	10511.C.PH.7.0	7	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CON
10511.C.PH.7.5	ACTAAGTACCCG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	Yes	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	C.PH_7.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_C.PH_7.5_F2	F2	10511.C.PH.7.5	171	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Not applicable	C	32.7157	116.1611	10511.C.PH.7.5	7.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CON
10511.C.PH.8.0	CTCAGCGGGACG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	Yes	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	C.PH_8.0	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_C.PH_8.0_G2	G2	10511.C.PH.8.0	172	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Not applicable	C	32.7157	116.1611	10511.C.PH.8.0	8	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CON
10511.C.PH.8.5	GTTAACTTACTA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	Yes	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	C.PH_8.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_C.PH_8.5_H2	H2	10511.C.PH.8.5	173	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Not applicable	C	32.7157	116.1611	10511.C.PH.8.5	8.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CON
10511.CF101.PH.5.0	TTGTGTCTCCCT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	CF101.PH_5.0	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_CF101.PH_5.0_A5	A5	10511.CF101.PH.5.0	174	per cent bubble in tube	26.87164091	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF101	32.7157	116.1611	10511.CF101.SPU	5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF101
10511.CF101.PH.5.5	TTCAGTTCGTTA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	CF101.PH_5.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_CF101.PH_5.5_B5	B5	10511.CF101.PH.5.5	175	per cent bubble in tube	22.23823397	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF101	32.7157	116.1611	10511.CF101.SPU	5.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF101
10511.CF101.PH.6.0	GACGTTAAGAAT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	CF101.PH_6.0	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_CF101.PH_6.0_C5	C5	10511.CF101.PH.6.0	176	per cent bubble in tube	26.57104327	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF101	32.7157	116.1611	10511.CF101.SPU	6	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF101
10511.CF101.PH.6.5	ATGGGACCTTCA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	CF101.PH_6.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_CF101.PH_6.5_D5	D5	10511.CF101.PH.6.5	177	per cent bubble in tube	28.17692997	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF101	32.7157	116.1611	10511.CF101.SPU	6.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF101
10511.CF101.PH.7.0	AGGGACTTCAAT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	CF101.PH_7.0	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_CF101.PH_7.0_E5	E5	10511.CF101.PH.7.0	178	per cent bubble in tube	13.2078422	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF101	32.7157	116.1611	10511.CF101.SPU	7	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF101
10511.CF101.PH.7.5	GTACGTCACTGA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	CF101.PH_7.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_CF101.PH_7.5_F5	F5	10511.CF101.PH.7.5	179	per cent bubble in tube	18.55232685	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF101	32.7157	116.1611	10511.CF101.SPU	7.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF101
10511.CF101.PH.8.0	CCGGCCGCGTGC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	CF101.PH_8.0	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_CF101.PH_8.0_G5	G5	10511.CF101.PH.8.0	180	per cent bubble in tube	10.66446906	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF101	32.7157	116.1611	10511.CF101.SPU	8	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF101
10511.CF101.PH.8.5	GCCGCCAGGGTC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	CF101.PH_8.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_CF101.PH_8.5_H5	H5	10511.CF101.PH.8.5	181	per cent bubble in tube	1.727537723	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF101	32.7157	116.1611	10511.CF101.SPU	8.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF101
10511.CF101.SPU	GAGCCATCTGTA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	CF101.SPU	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	Yes	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_CF101.SPU_C11	C11	10511.CF101.SPU	182	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF101	32.7157	116.1611	10511.CF101.SPU	Not applicable	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF101
10511.CF160.PH.5.0	CAATGTAGACAC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	CF160.PH_5.0	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_CF160.PH_5.0_A6	A6	10511.CF160.PH.5.0	183	per cent bubble in tube	27.44692925	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF160	32.7157	116.1611	10511.CF160.SPU	5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF160_1
10511.CF160.PH.5.5	CCGCGTCTCAAC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	CF160.PH_5.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_CF160.PH_5.5_B6	B6	10511.CF160.PH.5.5	184	per cent bubble in tube	36.17142521	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF160	32.7157	116.1611	10511.CF160.SPU	5.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF160_1
10511.CF160.PH.6.0	TGGTGGAGTTTC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	CF160.PH_6.0	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_CF160.PH_6.0_C6	C6	10511.CF160.PH.6.0	185	per cent bubble in tube	39.98057689	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF160	32.7157	116.1611	10511.CF160.SPU	6	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF160_1
10511.CF160.PH.6.5	TCCGATAATCGG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	CF160.PH_6.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_CF160.PH_6.5_D6	D6	10511.CF160.PH.6.5	186	per cent bubble in tube	52.79147184	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF160	32.7157	116.1611	10511.CF160.SPU	6.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF160_1
10511.CF160.PH.7.0	CGGCCAGAAGCA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	CF160.PH_7.0	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_CF160.PH_7.0_E6	E6	10511.CF160.PH.7.0	187	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF160	32.7157	116.1611	10511.CF160.SPU	7	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF160_1
10511.CF160.PH.7.5	TCGCTACAGATG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	CF160.PH_7.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_CF160.PH_7.5_F6	F6	10511.CF160.PH.7.5	188	per cent bubble in tube	12.26685166	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF160	32.7157	116.1611	10511.CF160.SPU	7.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF160_1
10511.CF160.PH.8.0	ATTATGATTATG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	CF160.PH_8.0	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_CF160.PH_8.0_G6	G6	10511.CF160.PH.8.0	189	per cent bubble in tube	4.460702976	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF160	32.7157	116.1611	10511.CF160.SPU	8	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF160_1
10511.CF160.PH.8.5	GCCACCGCCGGA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	CF160.PH_8.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_CF160.PH_8.5_H6	H6	10511.CF160.PH.8.5	190	per cent bubble in tube	0	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF160	32.7157	116.1611	10511.CF160.SPU	8.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF160_1
10511.CF160.SPU	TTCCACACGTGG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	CF160.SPU	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	Yes	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_CF160.SPU_D11	D11	10511.CF160.SPU	191	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF160	32.7157	116.1611	10511.CF160.SPU	Not applicable	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF160_1
10511.CF66.PH.5.0	GCGAGGAAGTCC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	CF66.PH_5.0	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_CF66.PH_5.0_A3	A3	10511.CF66.PH.5.0	192	per cent bubble in tube	1.872269607	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF66	32.7157	116.1611	10511.CF66.SPU.XTRA	5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF66
10511.CF66.PH.5.5	GGACAAGTGCGA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	CF66.PH_5.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_CF66.PH_5.5_B3	B3	10511.CF66.PH.5.5	193	per cent bubble in tube	5.623156342	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF66	32.7157	116.1611	10511.CF66.SPU.XTRA	5.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF66
10511.CF66.PH.6.0	CTGATGTACACG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	CF66.PH_6.0	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_CF66.PH_6.0_C3	C3	10511.CF66.PH.6.0	194	per cent bubble in tube	2.173913043	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF66	32.7157	116.1611	10511.CF66.SPU.XTRA	6	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF66
10511.CF66.PH.6.5	TGGAGCCTTGTC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	CF66.PH_6.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_CF66.PH_6.5_D3	D3	10511.CF66.PH.6.5	195	per cent bubble in tube	1.219429348	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF66	32.7157	116.1611	10511.CF66.SPU.XTRA	6.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF66
10511.CF66.PH.7.0	ACGTGCCTTAGA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	CF66.PH_7.0	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_CF66.PH_7.0_E3	E3	10511.CF66.PH.7.0	196	per cent bubble in tube	1.694800353	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF66	32.7157	116.1611	10511.CF66.SPU.XTRA	7	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF66
10511.CF66.PH.7.5	CACCCTTGCGAC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	CF66.PH_7.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_CF66.PH_7.5_F3	F3	10511.CF66.PH.7.5	197	per cent bubble in tube	1.685864448	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF66	32.7157	116.1611	10511.CF66.SPU.XTRA	7.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF66
10511.CF66.PH.8.0	ATGCCTCGTAAG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	CF66.PH_8.0	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_CF66.PH_8.0_G3	G3	10511.CF66.PH.8.0	198	per cent bubble in tube	1.502105223	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF66	32.7157	116.1611	10511.CF66.SPU.XTRA	8	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF66
10511.CF66.PH.8.5	GTTGTTCTGGGA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	CF66.PH_8.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_CF66.PH_8.5_H3	H3	10511.CF66.PH.8.5	199	per cent bubble in tube	0	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF66	32.7157	116.1611	10511.CF66.SPU.XTRA	8.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF66
10511.CF66.SPU.XTRA	GTCGCTTGCACA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	CF66.SPU.XTRA	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	Yes	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_CF66.SPU.XTRA_A11	A11	10511.CF66.SPU.XTRA	200	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF66	32.7157	116.1611	10511.CF66.SPU.XTRA	Not applicable	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF66
10511.CF94.PH.5.0	CAAATTCGGGAT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	CF94.PH_5.0	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_CF94.PH_5.0_A4	A4	10511.CF94.PH.5.0	201	per cent bubble in tube	11.91192992	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF94	32.7157	116.1611	10511.CF94.SPU	5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF94
10511.CF94.PH.5.5	TACGGTCTGGAT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	CF94.PH_5.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_CF94.PH_5.5_B4	B4	10511.CF94.PH.5.5	202	per cent bubble in tube	20.27134741	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF94	32.7157	116.1611	10511.CF94.SPU	5.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF94
10511.CF94.PH.6.0	ACGTATTCGAAG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	CF94.PH_6.0	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_CF94.PH_6.0_C4	C4	10511.CF94.PH.6.0	203	per cent bubble in tube	25.65365539	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF94	32.7157	116.1611	10511.CF94.SPU	6	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF94
10511.CF94.PH.6.5	TTACTTATCCGA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	CF94.PH_6.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_CF94.PH_6.5_D4	D4	10511.CF94.PH.6.5	204	per cent bubble in tube	31.73451855	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF94	32.7157	116.1611	10511.CF94.SPU	6.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF94
10511.CF94.PH.7.0	AGTTGTAGTCCG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	CF94.PH_7.0	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_CF94.PH_7.0_E4	E4	10511.CF94.PH.7.0	205	per cent bubble in tube	1.702417433	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF94	32.7157	116.1611	10511.CF94.SPU	7	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF94
10511.CF94.PH.7.5	GATGCCTAATGA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	CF94.PH_7.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_CF94.PH_7.5_F4	F4	10511.CF94.PH.7.5	206	per cent bubble in tube	2.085461355	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF94	32.7157	116.1611	10511.CF94.SPU	7.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF94
10511.CF94.PH.8.0	TTAGTTTGTCAC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	CF94.PH_8.0	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_CF94.PH_8.0_G4	G4	10511.CF94.PH.8.0	207	per cent bubble in tube	0	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF94	32.7157	116.1611	10511.CF94.SPU	8	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF94
10511.CF94.PH.8.5	AGGGTGACTTTA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	CF94.PH_8.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	No	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_CF94.PH_8.5_H4	H4	10511.CF94.PH.8.5	208	per cent bubble in tube	0	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF94	32.7157	116.1611	10511.CF94.SPU	8.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF94
10511.CF94.SPU	TCTTGCGGAGTC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	CF94.SPU	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	3	ROBE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate1	sequencing by synthesis	Yes	16S rRNA	V4	108379Z	208484Z	108364Z	RNBD9959	RQCF_Plate1_CF94.SPU_B11	B11	10511.CF94.SPU	209	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	No	CF94	32.7157	116.1611	10511.CF94.SPU	Not applicable	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	CF94
10511.H1.Spu	ATGCCGGTAATA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H1.Spu	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate69	sequencing by synthesis	Yes	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate69_H1.Spu_F7	F7	10511.H1.Spu	210	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H1	32.7157	116.1611	10511.H1.Spu	Not applicable	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H1
10511.H1.Spu.pH5	GGTCTTAGCACC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H1.Spu.pH5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H1.Spu.pH5_H2	H2	10511.H1.Spu.pH5	211	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H1	32.7157	116.1611	10511.H1.Spu	5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H1
10511.H1.Spu.pH5.5	TCTCGCACTGGA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H1.Spu.pH5.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H1.Spu.pH5.5_G2	G2	10511.H1.Spu.pH5.5	212	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H1	32.7157	116.1611	10511.H1.Spu	5.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H1
10511.H1.Spu.pH6	AGATGCAATGAT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H1.Spu.pH6	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H1.Spu.pH6_F2	F2	10511.H1.Spu.pH6	213	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H1	32.7157	116.1611	10511.H1.Spu	6	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H1
10511.H1.Spu.pH6.5	ACCCAAGCGTTA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H1.Spu.pH6.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H1.Spu.pH6.5_E2	E2	10511.H1.Spu.pH6.5	214	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H1	32.7157	116.1611	10511.H1.Spu	6.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H1
10511.H1.Spu.pH7	CAGGCCACTCTC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H1.Spu.pH7	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H1.Spu.pH7_D2	D2	10511.H1.Spu.pH7	215	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H1	32.7157	116.1611	10511.H1.Spu	7	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H1
10511.H1.Spu.pH7.5	ACTGTTTACTGT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H1.Spu.pH7.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H1.Spu.pH7.5_C2	C2	10511.H1.Spu.pH7.5	216	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H1	32.7157	116.1611	10511.H1.Spu	7.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H1
10511.H1.Spu.pH8	GTCCTACACAGC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H1.Spu.pH8	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H1.Spu.pH8_B2	B2	10511.H1.Spu.pH8	217	per cent bubble in tube	Not applicable	11/30/2015	TRUE	100	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H1	32.7157	116.1611	10511.H1.Spu	8	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H1
10511.H1.Spu.pH8.5	AAGTCTTATCTC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H1.Spu.pH8.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H1.Spu.pH8.5_A2	A2	10511.H1.Spu.pH8.5	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	101	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H1	32.7157	116.1611	10511.H1.Spu	8.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H1
10511.H2.Spu	CTGGTCTTACGG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H2.Spu	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate57	sequencing by synthesis	Yes	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate57_H2.Spu_E7	E7	10511.H2.Spu	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	102	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H2	32.7157	116.1611	10511.H2.Spu	Not applicable	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H2
10511.H2.Spu.pH5	GCCTACTCTCGG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H2.Spu.pH5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H2.Spu.pH5_H3	H3	10511.H2.Spu.pH5	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	103	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H2	32.7157	116.1611	10511.H2.Spu	5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H2
10511.H2.Spu.pH5.5	GCATTCGGCGTT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H2.Spu.pH5.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H2.Spu.pH5.5_F3	F3	10511.H2.Spu.pH5.5	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	104	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H2	32.7157	116.1611	10511.H2.Spu	5.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H2
10511.H2.Spu.pH6.5	ACTGGCAAACCT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H2.Spu.pH6.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H2.Spu.pH6.5_E3	E3	10511.H2.Spu.pH6.5	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	105	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H2	32.7157	116.1611	10511.H2.Spu	6	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H2
10511.H2.Spu.pH7	ACAGTTGTACGC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H2.Spu.pH7	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H2.Spu.pH7_D3	D3	10511.H2.Spu.pH7	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	106	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H2	32.7157	116.1611	10511.H2.Spu	6.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H2
10511.H2.Spu.pH7.5	GTCACGGACATT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H2.Spu.pH7.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H2.Spu.pH7.5_C3	C3	10511.H2.Spu.pH7.5	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	107	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H2	32.7157	116.1611	10511.H2.Spu	7	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H2
10511.H2.Spu.pH8	ATTTACAATTGA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H2.Spu.pH8	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H2.Spu.pH8_B3	B3	10511.H2.Spu.pH8	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	108	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H2	32.7157	116.1611	10511.H2.Spu	7.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H2
10511.H2.Spu.pH8.5a	TTGCACCGTCGA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H2.Spu.pH8.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H2.Spu.pH8.5_A3	A3	10511.H2.Spu.pH8.5a	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	109	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H2	32.7157	116.1611	10511.H2.Spu	8	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H2
10511.H2.Spu.pH8.5b	ACCAGTGACTCA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H2.Spu.pH8.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H2.Spu.pH8.5_G3	G3	10511.H2.Spu.pH8.5b	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	110	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H2	32.7157	116.1611	10511.H2.Spu	8.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H2
10511.H3.Spu	TATCCTGGTTTC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H3.Spu	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate45	sequencing by synthesis	Yes	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate45_H3.Spu_D7	D7	10511.H3.Spu	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	111	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H3	32.7157	116.1611	10511.H3.Spu	Not applicable	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H3
10511.H3.Spu.pH5	ACTGCCCGATAC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H3.Spu.pH5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H3.Spu.pH5_H4	H4	10511.H3.Spu.pH5	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	112	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H3	32.7157	116.1611	10511.H3.Spu	5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H3
10511.H3.Spu.pH5.5	TGGCGCACGGAC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H3.Spu.pH5.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H3.Spu.pH5.5_G4	G4	10511.H3.Spu.pH5.5	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	113	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H3	32.7157	116.1611	10511.H3.Spu	5.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H3
10511.H3.Spu.pH6	TCTACATACATA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H3.Spu.pH6	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H3.Spu.pH6_F4	F4	10511.H3.Spu.pH6	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	114	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H3	32.7157	116.1611	10511.H3.Spu	6	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H3
10511.H3.Spu.pH6.5	AACACCATCGAC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H3.Spu.pH6.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H3.Spu.pH6.5_E4	E4	10511.H3.Spu.pH6.5	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	115	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H3	32.7157	116.1611	10511.H3.Spu	6.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H3
10511.H3.Spu.pH7	ACCAGAAATGTC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H3.Spu.pH7	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H3.Spu.pH7_D4	D4	10511.H3.Spu.pH7	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	116	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H3	32.7157	116.1611	10511.H3.Spu	7	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H3
10511.H3.Spu.pH7.5	GAATATACCTGG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H3.Spu.pH7.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H3.Spu.pH7.5_C4	C4	10511.H3.Spu.pH7.5	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	117	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H3	32.7157	116.1611	10511.H3.Spu	7.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H3
10511.H3.Spu.pH8	CCACTGCCCACC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H3.Spu.pH8	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H3.Spu.pH8_B4	B4	10511.H3.Spu.pH8	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	118	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H3	32.7157	116.1611	10511.H3.Spu	8	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H3
10511.H3.Spu.pH8.5	CTCCGAACAACA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H3.Spu.pH8.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H3.Spu.pH8.5_A4	A4	10511.H3.Spu.pH8.5	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	119	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H3	32.7157	116.1611	10511.H3.Spu	8.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H3
10511.H4.Spu	AGTACGCAGTCT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H4.Spu	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate33	sequencing by synthesis	Yes	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate33_H4.Spu_C7	C7	10511.H4.Spu	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	120	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H4	32.7157	116.1611	10511.H4.Spu	Not applicable	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H4
10511.H4.Spu.pH5	TTCTTAACGCCT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H4.Spu.pH5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H4.Spu.pH5_H5	H5	10511.H4.Spu.pH5	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	121	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H4	32.7157	116.1611	10511.H4.Spu	5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H4
10511.H4.Spu.pH5.5	CATTTACATCAC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H4.Spu.pH5.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H4.Spu.pH5.5_G5	G5	10511.H4.Spu.pH5.5	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	122	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H4	32.7157	116.1611	10511.H4.Spu	5.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H4
10511.H4.Spu.pH6	GAGTCTTGGTAA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H4.Spu.pH6	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H4.Spu.pH6_F5	F5	10511.H4.Spu.pH6	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	123	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H4	32.7157	116.1611	10511.H4.Spu	6	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H4
10511.H4.Spu.pH6.5	TTATCCAGTCCT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H4.Spu.pH6.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H4.Spu.pH6.5_E5	E5	10511.H4.Spu.pH6.5	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	124	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H4	32.7157	116.1611	10511.H4.Spu	6.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H4
10511.H4.Spu.pH7	CTCATCATGTTC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H4.Spu.pH7	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H4.Spu.pH7_D5	D5	10511.H4.Spu.pH7	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	125	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H4	32.7157	116.1611	10511.H4.Spu	7	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H4
10511.H4.Spu.pH7.5	GAATCTGACAAC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H4.Spu.pH7.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H4.Spu.pH7.5_C5	C5	10511.H4.Spu.pH7.5	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	126	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H4	32.7157	116.1611	10511.H4.Spu	7.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H4
10511.H4.Spu.pH8	ATAGTTAGGGCT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H4.Spu.pH8	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H4.Spu.pH8_B5	B5	10511.H4.Spu.pH8	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	127	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H4	32.7157	116.1611	10511.H4.Spu	8	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H4
10511.H4.Spu.pH8.5	TCTGGCTACGAC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H4.Spu.pH8.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H4.Spu.pH8.5_A5	A5	10511.H4.Spu.pH8.5	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	128	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H4	32.7157	116.1611	10511.H4.Spu	8.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H4
10511.H5.Spu	GTTAGGGAGCGA	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H5.Spu	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate21	sequencing by synthesis	Yes	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate21_H5.Spu_B7	B7	10511.H5.Spu	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	129	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H5	32.7157	116.1611	10511.H5.Spu	Not applicable	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H5
10511.H5.Spu.pH5	CTCCCGAGCTCC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H5.Spu.pH5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H5.Spu.pH5_H6	H6	10511.H5.Spu.pH5	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	130	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H5	32.7157	116.1611	10511.H5.Spu	5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H5
10511.H5.Spu.pH5.5	GTGGGACTGCGC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H5.Spu.pH5.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H5.Spu.pH5.5_G6	G6	10511.H5.Spu.pH5.5	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	131	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H5	32.7157	116.1611	10511.H5.Spu	5.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H5
10511.H5.Spu.pH6	CAGTCTAGTACG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H5.Spu.pH6	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H5.Spu.pH6_F6	F6	10511.H5.Spu.pH6	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	132	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H5	32.7157	116.1611	10511.H5.Spu	6	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H5
10511.H5.Spu.pH6.5	GTTTATCTTAAG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H5.Spu.pH6.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H5.Spu.pH6.5_E6	E6	10511.H5.Spu.pH6.5	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	133	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H5	32.7157	116.1611	10511.H5.Spu	6.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H5
10511.H5.Spu.pH7	TTAGGATTCTAT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H5.Spu.pH7	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H5.Spu.pH7_D6	D6	10511.H5.Spu.pH7	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	134	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H5	32.7157	116.1611	10511.H5.Spu	7	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H5
10511.H5.Spu.pH7.5	ATTGCCTTGATT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H5.Spu.pH7.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H5.Spu.pH7.5_C6	C6	10511.H5.Spu.pH7.5	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	135	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H5	32.7157	116.1611	10511.H5.Spu	7.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H5
10511.H5.Spu.pH8	GACCCGTTTCGC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H5.Spu.pH8	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H5.Spu.pH8_B6	B6	10511.H5.Spu.pH8	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	136	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H5	32.7157	116.1611	10511.H5.Spu	8	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H5
10511.H5.Spu.pH8.5	AGTAGTTTCCTT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H5.Spu.pH8.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H5.Spu.pH8.5_A6	A6	10511.H5.Spu.pH8.5	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	137	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H5	32.7157	116.1611	10511.H5.Spu	8.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H5
10511.H6.Spu	CCTATTGCGGCC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF2	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H6.Spu	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	5	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate9	sequencing by synthesis	Yes	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate9_H6.Spu_A7	A7	10511.H6.Spu	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	138	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H6	32.7157	116.1611	10511.H6.Spu	Not applicable	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H6
10511.H6.Spu.pH5	TAGACTTCAGAG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H6.Spu.pH5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H6.Spu.pH5_H7	H7	10511.H6.Spu.pH5	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	139	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H6	32.7157	116.1611	10511.H6.Spu	5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H6
10511.H6.Spu.pH5.5	CGGCCTAAGTTC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H6.Spu.pH5.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H6.Spu.pH5.5_G7	G7	10511.H6.Spu.pH5.5	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	140	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H6	32.7157	116.1611	10511.H6.Spu	5.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H6
10511.H6.Spu.pH6	GTTCGAGTGAAT	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H6.Spu.pH6	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H6.Spu.pH6_F7	F7	10511.H6.Spu.pH6	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	141	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H6	32.7157	116.1611	10511.H6.Spu	6	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H6
10511.H6.Spu.pH6.5	GTTCGCCGCATC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H6.Spu.pH6.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H6.Spu.pH6.5_E7	E7	10511.H6.Spu.pH6.5	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	142	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H6	32.7157	116.1611	10511.H6.Spu	6.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H6
10511.H6.Spu.pH7	CAACGAACCATC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H6.Spu.pH7	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H6.Spu.pH7_D7	D7	10511.H6.Spu.pH7	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	143	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H6	32.7157	116.1611	10511.H6.Spu	7	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H6
10511.H6.Spu.pH7.5	GAGCCCAAAGAG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H6.Spu.pH7.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H6.Spu.pH7.5_C7	C7	10511.H6.Spu.pH7.5	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	144	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H6	32.7157	116.1611	10511.H6.Spu	7.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H6
10511.H6.Spu.pH8	TGACTGCGTTAG	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H6.Spu.pH8	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H6.Spu.pH8_B7	B7	10511.H6.Spu.pH8	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	145	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H6	32.7157	116.1611	10511.H6.Spu	8	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H6
10511.H6.Spu.pH8.5	CAGATCCCAACC	GTGTGCCAGCMGCCGCGGTAA	No	UCSDMI	RQCF_cultures	No	cf sputum cultured at various ph's to determine the effect on bacterial growth	HOWE_KF1	PM16B1	Illumina_MiSeq	Illumina MiSeq V4 515fbc/806r EMP protocol	GT	151040311	H6.Spu.pH8.5	FWD:GTGCCAGCMGCCGCGGTAA; REV:CCGTCAATTCCTTTRAGTTT	Illumina	LDG	30616	4	RIKE	RQCF	UCSDMI	5/4/16	RQCF_AG_S1_L001	RQCF_Plate2	sequencing by synthesis	No	16S rRNA	V4	108379Z	311318B	311426B	RNBD9959	RQCF_Plate2_H6.Spu.pH8.5_A7	A7	10511.H6.Spu.pH8.5	Missing: Not provided	per cent bubble in tube	Not applicable	11/30/2015	TRUE	146	urban biome	research facility	biofilm	microbial mat/biofilm	USA:CA:San Diego	Yes	H6	32.7157	116.1611	10511.H6.Spu	8.5	Dorrestein UCSDMI	TRUE	1	biofilm	biofilm metagenome	718308	pH Effects on Cystic Fibrosis Microbiome	H6
